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Area calcified in mouse Achilles tendons, as revealed with Alizarin Red staining. Tendons from old mice were extensively calcified, whereas tendons from young mice showed minimal calcification. Rapamycin-fed mice exhibited minimal tendon calcification equal to that from tendons of adult mice. Scale bar = 250 μm. Data are presented as means ± SEM. *Significantly different from adult. #Significantly different from old.
