Figure 5.

The effect of rapamycin (RAPA) on mouse Achilles tendon gene expression of proteins important in bone and cartilage formation. Compared with age-matched controls, tendons from RAPA-fed mice showed decreased expression of the chondrocyte and osteoblast markers (A) Sox9 and (B) Sp7, respectively. RAPA also attenuated the expression of the bone marker (C) Bglap. (D) Expression of the hypoxia marker Hif1a was unchanged with RAPA. Data are presented relative to adult expression levels and are presented as means ± SEM. *Statistically different from adult. ^#Statistically different from old.