Figure 1.

Intermittent administration of rapamycin extends life span. (A) Kaplan–Meier plot showing life span of C57BL/6J.Nia female mice administered rapamycin (2mg/kg) or vehicle once every 5 days (n = 20 vehicle-treated mice, 14 rapamycin-treated mice; p = .002, one-tailed log-rank test). Mean, median, and maximum (75th percentile, p = .005, one-tailed Boschloo exact test) life span are shown; this analysis excludes six rapamycin-treated mice that developed dermatitis. (B) Life span analysis excluding only three rapamycin-treated mice euthanized due to dermatitis (n = 20 vehicle-treated mice, 17 rapamycin-treated mice; p = .011, one-tailed log-rank test; 75th percentile life span p = .01, one-tailed Boschloo exact test). (C) Life span analysis inclusive of every mouse that received vehicle or rapamycin, included those euthanized because of dermatitis (n = 20 vehicle-treated mice, 20 rapamycin-treated mice; p = .035, one-tailed log-rank test; 75th percentile life span p = .02, one-tailed Boschloo exact test).