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. 2016 Jun 14;6:27724. doi: 10.1038/srep27724

Figure 4. Influence of CGRP or CGRP 8–37 on HUMSCs apoptosis, death and proliferation.

Figure 4

(A) Apoptotic and dead cells were quantified through FACS analysis after staining with Annexin-V and PI. The Annexin-V+/PI− cells appeared early in the apoptotic process. Cells in death or late apoptosis were Annexin- V+/PI+. (B) The percentage of apoptotic and dead cells in CGRP or CGRP 8–37 groups compared with control. Cells in control group were cultured in only L-DMEM containing 10% FBS. (C) The CCK-8 proliferation assay showed the OD value after treatment with CGRP or CGRP 8–37 for 7 days. OD value = OD (450 nm)–OD (650 nm). Data are shown as mean ± SEM from at least three independent experiments.