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. 2016 Jun 14;6:27724. doi: 10.1038/srep27724

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Copyright © 2016, Macmillan Publishers Limited

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HUMSCs were stimulated with various concentrations (0, 10⁻⁶, 10⁻⁷, 10⁻⁸ and 10⁻⁹ mol/L) of CGRP for 6 h. The active phosphorylated forms of ERK1/2, Akt, p38MAPK and SAPK/JNK (p-ERK1/2, p-Akt, p-p38MAPK, and p-SAPK/JNK) and the total proteins (ERK1/2, Akt, p38MAPK, and SAPK/JNK) were measured by densitometry using the Image Lab 4.1 software. The density ratio of phosphorylated to total ERK1/2, Akt, p38MAPK and SAPK/JNK was normalized to its related control. Equal protein loading was assessed with β-actin levels. Data are presented as the mean ± SEM. *P < 0.05 and **P < 0.01 compared with the respective CGRP-unstimulated HUMSCs.