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. 2016 Jun 14;6:27705. doi: 10.1038/srep27705

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Phylogenetic comparison of DOF protein sequences from Arabidopsis thaliana, Brassica napus, Oryza sativa, Capsella rubella, Camelina sativa and Eutrema salsugineum in the database. Alignments were made by ClustalW2 and MEGA5.1 software using the default parameters and excluding positions with gaps. To determine OBP4 expression in response to hormone treatment, eight-day-old seedlings were transferred to fresh medium (mock), or medium that contained (B) IAA (0.25 μM) (C) GA (0.25 μM) (D) JA (0.25 μM) (E) SA (0.5 μM) (F) ABA (0.1 μM) or (G) CK (0.1 μM) for the indicated period. RNA was then isolated from whole seedlings and analysed. Three biological and technical replicates were used. Asterisks indicate significant differences, p < 0.05.