Table 1.
Effects of Wolbachia on ZIKV Prevalence
| Isolate | ZIKV Titer (PFU/mL) | Days Post-infection |
wMel_Br |
Br |
wMel_Br |
Br |
wMel_Br |
Br |
|---|---|---|---|---|---|---|---|---|
| Head/Thorax Infection Rate | Abdomen Infection Rate | Saliva Infection Rate | ||||||
| BRPE | 5.0 × 106 | 7 | 0 | 65 | 55 | 85 | – | – |
| 14 | 10 | 100 | 35 | 100 | 45 | 100 | ||
| SPH | 8.7 × 103 | 7 | 5 | 95 | 30 | 90 | – | – |
| 14 | 25 | 95 | 30 | 95 | – | – | ||
Ae. aegypti were orally infected with fresh, low-passage ZIKV. Initial viral titer was determined by plaque-forming assay. Saliva infection was only examined for mosquitoes at 14 days post-infection with the BRPE isolate. Infection rates are given as percentages. n = 20 per group unless specified. ZIKV, Zika virus; PFU, plaque-forming units; BRPE, ZIKV/H. sapiens/Brazil/BRPE243/2015; SPH, ZIKV/H. sapiens/Brazil/SPH/2015; wMel_Br, Wolbachia-infected; Br, Wolbachia-uninfected.