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. 2016 Apr 29;2(6):427–441. doi: 10.1021/acsinfecdis.6b00034

Figure 2.

Figure 2

YnMyr labels proteins in T. brucei BSF parasites. (a) Time-dependent metabolic incorporation of 100 μM YnMyr or Myr (−) in BSF parasites. (b) Phenotype of YnMyr treatment at 18 h in BSF. Scale bar = 10 μm. See also Supporting Information Figure S3. (b) NaOH treatment of lysates from BSF parasites incubated with YnMyr reveal labeling of a base sensitive band at ∼60 kDa. (d) Chemical structures of myristate analogues YnMyr and AzMyr and palmitate analogue YnPal. (e) Comparative labeling with different fatty acid analogues at 100 μM in BSF. Coomassie gels are shown in Supporting Information Figure S2. Additional data are shown in Supporting Information Figure S4.