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Ni-affinity nanogold particle revealed the location of AML1-175 on TRiC. (A) Representative area of a micrograph of a vitrified sample of AML1-175-TRiC-Ni-nanogold complex. (B) Gallery of selected images, class averages, and projection of AML1-175-TRiC-Ni-nanogold complex. (C) Top, bottom, and side views of the 3D reconstruction of TRiC-AML1-175 labeled with Ni-nanogold. The density from nanogold particles is colored in gold and the black arrowheads indicate the protruding subunit.
