Skip to main content
. 2016 Mar 18;30(6):877–887. doi: 10.1038/eye.2016.43

Figure 1.

Figure 1

Schematic representation of the GV120 plasmid and the efficacy of Ad-S100A4-RNAi transfer into the mouse retina. (a) ITR, inverted terminal repeat; PhU6, human U6 promoter; MCS, multiple cloning site; AgeI, restriction enzyme; EcoRI, restriction enzyme; PUbi, ubiquitin promoter; GFP, green fluorescent protein; Poly A, SV40 polyadenylation signal; Ori, Escherichia coli origin of replication; Amp, ampicillin resistance sequence. Ad: mouse S100A4 RNAi coding sequence. (b1, b2) The Ad-S100A4-RNAi transferred into the retina of a P12 C57BL/6J mouse 5 days after the intravitreal injection of ~1.0 × 109 plaque-forming units of Ad-S100A4-RNAi. Retinas were obtained from the control group and OIR-S100A4-RNAi group mice for cryosection, which were immunostained with an antibody for GFP and observed using fluorescence microscopy. Fluorescence of GFP can hardly be seen in retina cryosections from the control group (b1) (treated with nothing) but can be easily observed in the ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), and the outer plexiform layer (OPL), and less easily observed in the outer nuclear layer (ONL) of retinas from the OIR-S100A4-RNAi group (b2) (treated with Ad-S100A4-RNAi). Images b1 and b2 were taken at 400 × magnification for optimal comparison; scale bars=50 μm.