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. 2016 Mar 18;30(6):877–887. doi: 10.1038/eye.2016.43

Figure 5.

Figure 5

Effects of Ad-S100A4-RNAi transfer on the retinal protein levels of BDNF and VEGF. (a, b) Retinal protein levels of BDNF, VEGF, and HIF-1α were measured by western blot analysis, which demonstrated that the protein levels of BDNF, VEGF, and HIF-1α were downregulated in OIR-S100A4-RNAi group than those in OIR and OIR-GFP group. BDNF: OIR-S100A4-RNAi group vs OIR group, ***P<0.05; OIR-S100A4-RNAi group vs OIR-GFP group, ***P<0.05; VEGF: OIR-S100A4-RNAi group vs OIR group, ***P<0.05; OIR-S100A4-RNAi group vs OIR-GFP group, ***P<0.05. HIF-1α: OIR-S100A4-RNAi group vs OIR group, ***P<0.05; OIR-S100A4-RNAi group vs OIR-GFP, group ***P<0.05. (c) Real-time PCR analysis of BDNF, VEGF, and HIF-1α expression further confirmed that mRNA levels of BDNF, VEGF, and HIF-1α significantly decreased in the OIR-S100A4-RNAi group compared with the OIR and OIR-GFP groups. BDNF: OIR-S100A4-RNAi group vs the OIR group, *P<0.05; OIR-S100A4-RNAi group vs OIR-GFP group, **P<0.05. VEGF: OIR-S100A4-RNAi group vs OIR group, *P<0.05; OIR-S100A4-RNAi group vs OIR-GFP group, *P<0.05. HIF-1α: OIR-S100A4-RNAi group vs OIR group, **P<0.05; OIR-S100A4-RNAi group vs OIR-GFP group, *P<0.05.