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. 2016 May 18;12(1):114–120. doi: 10.3892/ol.2016.4611

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Copyright: © Guan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Immunohistochemical staining of Emi1 and Ki-67 in ESCC tissues. Paraffin-embedded tissue sections were stained with antibodies against Emi1 and Ki-67, and counterstained with hematoxylin.(A and B) Emi1 reactivity (magnification, ×400). (C and D) Ki-67 staining (SP ×400). (A and C) Well differentiated ESCC specimens displayed (A) weak Emi1 and (C) weak Ki-67 immunostaining. (B and D) Moderate/poor differentiated ESCC tissues exhibited (B) strong Emi1 (B) and (D) strong brown nuclear Ki-67 immunostaining. ESCC, esophageal squamous cell carcinoma; Emi1, early mitotic inhibitor-1.