Table 2.
Protein differentially expressed for Shaziling pig breeds and Yorkshire were identified by 2-DE and MALDI-TOF-MS/MS
| Sport no | NCBI accession number | Protein name | Mr, kDa theor | pI theor | No. of peptides identified | Mascot score | Sequence coverage | Expecte |
|---|---|---|---|---|---|---|---|---|
| Up-regulated in Yorkshire pigs | ||||||||
| 1375 | gi|89574051 | mitochondrial ATP synthase, H+ transporting F1 complex beta subunit, partial, ATP5B | 47059.6 | 4.99 | 16 | 1180 | 46 % | 5.8e-11 |
| 1929 | gi|283993079 | L-gulonate3-dehydrogenase | 35433.2 | 5.79 | 14 | 417 | 51 % | 1.2e-03 |
| 2268 | gi|545835136 | PREDICTED: NADH dehydrogenase ubiquinone flavoprotein 2 isoform X1 | 25812.2 | 6.96 | 14 | 435 | 60 % | 1.8e-039 |
| 2247 | gi|55926209 | heat shock protein beta-1 | 22984.7 | 6.23 | 9 | 423 | 41 % | 2.9e-03 |
| 2393 | gi|809283 | Chain B, Structure Determination Of Aquomet Porcine Hemoglobin At 2.8 Angstrom Resolution | 16082.4 | 6.76 | 12 | 511 | 86 % | 4.6e-04 |
| 2377 | gi|545848507 | PREDICTED: alpha-crystallin B chain isoform X2 | 20116.4 | 6.76 | 9 | 212 | 51 % | 3.7e-01 |
| 2497 | gi|494389 | Chain A, High Resolution X-Ray Structures Of Pig Metmyoglobin | 16901.8 | 6.5 | 9 | 529 | 59 % | 7.3e-04 |
| 2500 | gi|494389 | Chain A, High Resolution X-Ray Structures Of Pig Metmyoglobin | 16901.8 | 6.5 | 12 | 596 | 82 % | 1.5e-05 |
| 2575 | gi|55926217 | cytochrome c oxidase subunit 5B, mitochondrial precursor | 14002 | 8.8 | 8 | 334 | 46 % | 2.3e-02 |
| 2594 | gi|809283 | Chain B, Structure Determination Of Aquomet Porcine Hemoglobin At 2.8 Angstrom Resolution | 16082.4 | 6.76 | 9 | 400 | 67 % | 5.8e-03 |
| Up-regulated in Shaziling pigs | ||||||||
| 2480 | gi|117660856 | MYLPF | 19.0 | 4.8 | 6 | 110 | 36 % | 5.8e-00 |
| 2395 | gi|117660874 | MLC1f | 21018.6 | 4.9 | 11 | 444 | 60 % | 2.3e-40 |
| 2654 | gi|117660856 | MYLPF | 19066.3 | 4.8 | 9 | 347 | 49 % | 1.2e-03 |
| 2422 | gi|117660890 | MLC3f | 16761.2 | 4.6 | 4 | 250 | 29 % | 2.3e-02 |
| 2659 | gi|117660856 | MYLPF | 19066.3 | 4.8 | 6 | 144 | 27 % | 2.3e-01 |
| 2400 | gi|545858131 | keratin, type I cytoskeletal 10 isoform X2 | 58318.6 | 4.9 | 16 | 190 | 21 % | 5.8e-01 |
| 2482 | gi|117660856 | MYLPF | 19066.3 | 4.8 | 16 | 600 | 86 % | 5.8e-05 |
| 2522 | gi|117660874 | MLC1f | 21018.6 | 4.9 | 8 | 190 | 40 % | 5.8e-01 |
| 1154 | gi|545845559 | PREDICTED: fibrinogen beta chain isoform X2 | 50399.8 | 7.94 | 18 | gi|545845559 | 43 % | 4.6e-03 |
| 2225 | gi|262263205 | triosephosphate isomerase 1 | 26878.9 | 6.54 | 14 | 463 | 74 % | 2.9e-04 |
| 2203 | gi|262263205 | Triosephosphate somerase 1 | 26878.9 | 6.54 | 17 | 774 | 77 % | 2.3e-07 |
| 1326 | gi|311247991 | PREDICTED: pyruvate dehydrogenase protein Xcomponent-like isoform | 54036.4 | 8.3 | 12 | 317 | 28 % | 1.2e-02 |
| 1416 | gi|545801458 | PREDICTED: actin, alpha cardiac muscle 1 isoform X1 | 42334 | 5.2 | 10 | 313 | 31 % | 2.9e-02 |
| 2250 | gi|262263205 | Triosephosphate isomerase 1,TPI1 | 26878.9 | 6.5 | 17 | 795 | 82 % | 1.8e-07 |
| 2584 | gi|314907119 | A-FABP adipocyte fatty acid-binding protein | 14780.6 | 6.2 | 8 | 371 | 56 % | 4.6e-03 |
| 1433 | gi|545833443 | PREDICTED: alpha-enolase isoform X1 | 38172.7 | 8.93 | 12 | 377 | 41 % | 1.2e-03 |
| 2221 | gi|55926209 | HSPB1 heat shock protein beta-1 | 22984.7 | 6.23 | 8 | 236 | 31 % | 1.5e-01 |
| 1805 | gi|545859898 | PREDICTED: beta-enolase isoform X1 | 48154.9 | 8.52 | 17 | 414 | 40 % | 2.3e-03 |
| 2550 | gi|545805333 | PREDICTED: 14 kDa phosphohistidine phosphatase isoform X2 | 14036.8 | 5.91 | 14 | 454 | 74 % | 2.3e-04 |
| 2217 | gi|262263205 | triosephosphate isomerase 1 | 26878.9 | 6.54 | 16 | 701 | 82 % | 4.6e-06 |
| 1711 | gi|46389777 | troponin T fast skeletal muscle type | 30720.1 | 8.68 | 14 | 423 | 41 % | 2.9e-03 |
| 1137 | gi|545845559 | PREDICTED: fibrinogen beta chain isoform X2 | 50399.8 | 7.94 | 15 | 289 | 34 % | 7.3e-02 |
| 2215 | gi|262263205 | triosephosphate isomerase 1 | 26878.9 | 6.54 | 18 | 684 | 76 % | 2.3e-06 |
| 2223 | gi|55926209 | HSPB1 heat shock protein beta-1 | 22984.7 | 6.23 | 10 | 371 | 42 % | 4.6e-03 |
| 1833 | gi|545832797 | PREDICTED: troponin T, slow skeletal muscle isoformX1 | 32449.6 | 5.54 | 9 | 418 | 32 % | 9.2e-03 |
| 1374 | gi|268607671 | actin, alpha skeletal muscle | 42366 | 5.23 | 11 | 348 | 35 % | 9.2e-03 |
eNumber of times we would expect to obtain an equal or higher score by chance