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. 2016 Jun 14;14:175. doi: 10.1186/s12967-016-0932-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Linearity of the IFNγ/IL2 FluoroSpot assay. Magnitude of IFNγ, IL2, and IFNγ + IL2 responses of donor S25 (a) and S26 (b) within the IFNγ/IL2 FluoroSpot assay as a function of cell density. Depicted are the number of mitogen-specific IFNγ, IL2 and IFNγ + IL2 SFC/well after stimulation with 2 µg/ml PHA-L; SFC spot forming cells. R ² = coefficient of determination