Table 3.
Days post exposure | Calf | Medial retropharyngeal lymph node | Mesenteric lymph node | Lung | Ileum | Colon |
---|---|---|---|---|---|---|
22 | E4 | 6.9 | 6.3 | Not done | Not done | Not done |
28 | E2 | 6.7 | Negative | Not done | Not done | Not done |
35 | E3 | Negative | 5.0 | Negative | 6.0 | 5.2 |
42 | E1 | 6.2 | 7.4 | Negative | 7.0 | 6.0 |
Tissue samples from lymph nodes, lung, Ileum and Colon were harvested from exposed group calves euthanized at the indicated number of days after exposure to field group calves. The number of viral RNA copies (VRC) of BCoV was quantified with RT-qPCR and the limit of quantification was 4.2 log10 VRC/g tissue