Table 5.
Primers used for qRT-PCR validation
| Gene | Primer Sequence (5′-3′) |
|---|---|
| Cytochrome b | Cytb-F: TGAGCCGCAACAGTAATC |
| Cytb-R: AAGGGAAAAGGAAGTGAAAG | |
| Complement component 3 | AjC3-F: GCGTTGTTTCGTTCAACAAGGGGA |
| AjC3-R: GCCATTCACTGGAGGTGTGCCA | |
| Complement factor B | Bf-F: ATTATCTCGCAACAGCGATCC |
| Bf-R: GGGCAACCACACCGGCTTCTCCA | |
| IRAK4-like | IRAK4-F: TACACGTCAGATCGGGATGA |
| IRAK4-R: TAAACGACGAGCGTACCACA | |
| NF-kB transcription factor Rel | Rel-F: TGCGAAGCCACATCCATT |
| Rel-R: AGGGCATCCTTTAAGTCAGC | |
| MAPKKK | MAPKKK -F: GAATCAGAGGAGATAGATGTGGAGA |
| MAPKKK -R: AGGAGGAGGAGGAAGACGAC | |
| Serine/threonine-protein kinase TBK1-like | TBK1-F: AGATGATGTTGTCCATTCTCG |
| TBK1-R: ACAGGAGGAAGTGATGTGCT | |
| TGF beta-activated kinase | TAK -F: TCTCTGTAGCCTCCTTTGACG |
| TAK -R: CTCGGTCTTCCAACCAACAC | |
| BIRC2 | BIRC2-F: TCAGGCACGAGTGACAAAGT |
| BIRC2-R: GCATGAGCCATTCACATCTCA | |
| NF-kB p105 subunit | p105-F: GCAACACACCCCTCCATCTT |
| p105-R: TCTTCTTCGCTAACGTCACACC | |
| MyD88 | MyD88-F: CCGATGTAGGAGGATGGTAGTAG |
| MyD88-R: CACAGTAAGGTGCTGAAGAATGC | |
| HSP 70 | HSP 70-F: AAGAGCACAGGCAAAGAG |
| HSP 70-R: TGATGATGGGTTGGCACA | |
| HSP 90 | HSP 90-F: TATGAAAGCCTGACAGACGCAAGC |
| HSP 90-R: TAACGCAGAGTAAAAGCCAACACC | |
| Matrix metalloproteinase-24 | MMP-F: CGATTCAGTCTTCCCTGGTG |
| MMP-R: ACCGTCATCAACTTTCCTGGT | |
| Apoptosis regulator BAX-like | BAX-F: GCCGTGGGACTGACTTTACA |
| BAX-R: TCCATCTCGTAGTTCTCTCAACG | |
| Thioredoxin | TRx-F: GCTGGTGACAAACTGGTGAT |
| TRx -R: TGAGAAAGACAACGTCGGTA | |
| CLECT | CLECT-F: GACGGCTTGTCCAGAGTT |
| CLECT-R: AGGTCCATTGTTGGGTTC | |
| CLEC19A | CLEC19A-F: ATGCAGCGAGAAGATGGAGT |
| CLEC19A-R: TGGCAGGATATGCCCTAGAT | |
| GalNAc-specific lectin | GalNAc -F: CCATCCTTCAGGGCAGATAA |
| GalNAc -R: TTCATCGACCAAAATGCAGA | |
| Major yolk protein | MYP-F: AGGAGGGAGACATTGCTT |
| MYP-R: ATGATGCTTTCTGGGTTG | |
| PDRG 1 | PDRG1-F: AATTGGAGGAACTCGCTGAA |
| PDRG1-R: TTGCTTATCGCCTTCTTGTG |