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. 2016 May 17;12(1):323–330. doi: 10.3892/ol.2016.4597

Figure 6.

Figure 6.

PathScan® Intracellular Array analysis. Metastatic mouse breast cancer 4T1 cells were treated with 10 nM bor, 100 nM bor, 1 µM BAPTA-AM, 5 µM BP alone or 10 nM bor + 1 µM BP, 10 nM bor + 5 µM BP, 100 nM bor + 1 µM BP or 100 nM bor + 5 µM BP at the logarithmic phase of growth for 24 h. Following treatment, the cells were analyzed using the PathScan® Intracellular Signalling Array kit, according to the manufacturer's protocol. The slide was incubated with Lumi-GLO/peroxide reagent and exposed to Kodak BioMax X-ray films in dark room. The result is representative of two experiments, each run in duplicate. BP, BAPTA-AM; bor, bortezomib; SAPK/JNK, stress-activated protein and Jun amino-terminal kinases.