Figure 6. Interaction of endogenous c-FOS with DBH promoter variant C-2073T in cella: ChIP (Chromatin ImmunoPrecipitation). Results of nucleosomal immunoprecipitates from PC12 cells. “Input” DNA derives from the nucleosomal preparation before immunoprecipitation. Antibodies directed against c-FOS (or preimmune serum, “mock”) were used to precipitate nucleosomes derived from PC12 cells transfected with either the C-2073 or −2073T alleles, on promoter/reporter plasmids. The nucleosomal immunoprecipitates were interrogated with PCR primers producing a 161-bp amplicon spanning C-2073T. DBH, dopamine β-hydroxylase.
