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. 2016 Jun 13;16:108. doi: 10.1186/s12866-016-0723-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a Agarose gel (2 %) showing DNA detection limits of C-PCR and N-PCR by amplifying fliC-d gene for typhoid diagnosis. Lane 1 to lane 8, diluted bacterial DNA (10-fold) from 3 × 10⁶ to 3 × 10⁻¹copies/reaction;lane 3, 3 × 10⁴copies/reaction (sensitivity of C-PCR); lane 7, 3 × 10⁰copies/reaction (sensitivity of N-PCR); lane 9, positive control (S. Typhi CT18); lane 10, negative control (sterile distilled water); lane 11, 100-bp DNA ladder marker. b Determination of analytical sensitivity of Q-PCR. A standard curve was generated by plotting the threshold cycles (Ct) values on y axis and DNA copy numbers/reaction on x axis. The dashed line indicates the Ct cutoff value of ≤37 at 3 × 10⁰ copies/reaction (sensitivity of Q-PCR)