Table I.
Primer sequences for the polymerase chain reaction amplification of AmpC β-lactamase target genes.
| Target gene | Primer sequence (5′→3′) | Fragment length (bp) |
|---|---|---|
| blaMOX | F: GCTGCTCAAGGAGCACAGGAT | 520 |
| R: CACATTGACATAGGTGTGGTGC | ||
| blaCIT | F: TGGCCAGAACTGACAGGCAAA | 462 |
| R: TTTCTCCTGAACGTGGCTGGC | ||
| blaDHA | F: AACTTTCACAGGTGTGCTGGGT | 405 |
| R: CCGTACGCATACTGGCTTTGC | ||
| blaACC | F: AACAGCCTCAGCAGCCGGTTA | 346 |
| R: TTCGCCGCAATCATCCCTAGC | ||
| blaEBC | F: TCGGTAAAGCCGATGTTGCGG | 302 |
| R: CTTCCACTGCGGCTGCCAGTT | ||
| blaFOX | F: AACATGGGGTATCAGGGAGATG | 190 |
| R: CAAAGCGCGTAACCGGATTGG | ||
| blaDHA (full-length primer) | F: GGAATTCCACGGAAGGTTAATTCTGATG | 1140 |
| R: GCAAGCTTTTATTCCAGTGCACTCA |
F, forward; R, reverse.