Table I.
Results of preliminary range-finding tests in the bacterial reverse mutation assay.
| Colonies/plate, mean (factor)a | ||||||
|---|---|---|---|---|---|---|
| Treatment | Dose (µg/plate) | TA98 | TA100 | TA1535 | TA1537 | WP2uvrA |
| Without S9 | ||||||
| Vehicle controlb | 29 (1.00) | 127 (1.00) | 14 (1.00) | 11 (1.00) | 21 (1.00) | |
| PR extract | 0.05 | 27 (0.93) | 119 (0.94) | 15 (1.07) | 8 (0.73) | 19 (0.90) |
| 0.5 | 26 (0.90) | 132 (1.04) | 12 (0.86) | 9 (0.82) | 20 (0.95) | |
| 5 | 30 (1.03) | 125 (0.98) | 13 (0.93) | 10 (0.91) | 21 (1.00) | |
| 50 | 29 (1.00) | 106 (0.83) | 12 (0.86) | 11 (1.00) | 17 (0.81) | |
| 500 | 23 (0.79) | 98 (0.77) | 9 (0.64) | 7 (0.64) | 16 (0.76) | |
| 1,000 | 10 (0.34) | 51 (0.40) | 5 (0.36) | 5 (0.45) | 9 (0.43) | |
| 5,000 | 8 (0.28) | 38 (0.30) | 3 (0.21) | 3 (0.27) | 4 (0.19) | |
| Sodium azide | 0.5 | 398 (3.13) | 483 (34.50) | |||
| 4-Nitroquinolone-1-oxide | 0.5 | 282 (9.72) | 132 (6.29) | |||
| 9-Aminoacridine | 50 | 295 (26.82) | ||||
| With S9 | ||||||
| Vehicle controlb | 38 (1.00) | 119 (1.00) | 9 (1.00) | 17 (1.00) | 17 (1.00) | |
| PR extract | 0.05 | 36 (0.95) | 121 (1.02) | 7 (0.78) | 16 (0.94) | 16 (0.94) |
| 0.5 | 40 (1.05) | 117 (0.98) | 9 (1.00) | 18 (1.06) | 14 (0.82) | |
| 5 | 32 (1.84) | 108 (0.91) | 10 (1.11) | 17 (1.00) | 13 (0.76) | |
| 50 | 35 (0.92) | 105 (0.88) | 8 (0.89) | 15 (0.88) | 18 (1.06) | |
| 500 | 30 (0.79) | 93 (0.78) | 6 (0.67) | 11 (0.65) | 15 (0.88) | |
| 1,000 | 15 (0.39) | 42 (0.35) | 3 (0.33) | 6 (0.35) | 7 (0.41) | |
| 5,000 | 7 (0.18) | 32 (0.27) | 2 (0.22) | 4 (0.24) | 4 (0.24) | |
| 2-Aminoanthracene | 0.4 | 406 (11.28) | 252 (2.08) | |||
| 2 | 327 (46.71) | 351 (21.94) | ||||
| 4 | 223 (13.94) | |||||
a
Factor is the number of colonies of the treated plate/number of colonies of the negative control plate.
b
Vehicle control, sterile distilled water. PR, Persicariae Rhizoma.