Table III.

Results of preliminary range-finding tests for the chromosomal aberration assay.

Treatment conditions	Dose (µg/ml)	Viable cells/flask	RCCa (%)
Treatment time 6 h, recovery time 18 h, with S9
Vehicle controlb		11,871.25±1,034.27	100.00
PR extract	0.05	11,327.00±941.60	95.42
	0.5	12,054.25±1,123.84	101.54
	5	11,482.50±871.57	96.73
	50	11,931.50±568.58	100.51
	500	12,085.25±77.74	101.80
	1,000	10,641.25±691.62	89.64
	5,000	11,292.75±975.90	95.13
Cyclophosphamide	6	7,694.00±822.28	64.81
Treatment time 6 h, recovery time 18 h, without S9
Vehicle controlb		11,954.00±2,032.45	100.00
PR extract	0.05	11,469.00±852.76	95.94
	0.5	11,859.50±1,540.14	99.21
	5	11,902.75±1,909.86	99.57
	50	12,344.00±1,063.40	103.26
	500	11,473.75±895.79	95.98
	1,000	12,118.00±1,116.35	101.37
	5,000	10,836.00±436.41	90.65
Ethylmethanesulfonate	800	7,609.75±465.86	63.66
Treatment time 24 h, recovery time 0 h, without S9
Vehicle controlb		11,220.75±978.78	100.00
PR extract	0.05	10,062.50±882.62	89.68
	0.5	11,643.25±1,526.13	103.77
	5	11,900.75±556.52	106.06
	50	11,304.50±2,231.19	100.75
	500	10,628.25±519.86	94.63
	1,000	11,380.00±2,133.52	101.42
	5,000	12,308.25±1,459.35	109.69
Ethylmethanesulfonate	600	7,019.75±1,146.26	62.56

Values of viable cells/flask are expressed as mean ± standard deviation of two independent experiments.

^aRCC, relative cell count (%) = (cell count of treated flask/cell count of control flask × 100.

^bVehicle control, sterile distilled water. PR, Persicariae Rhizoma.