Table IV.

Results of the chromosomal aberration assay.

		Aberrant metaphases		Total aberrations
Treatment conditions	Dose (µg/ml)	With gaps	Without gaps	With gaps	Without gaps	PP	ER
Treatment time 6 h, recovery time 18 h, with S9
Vehicle controla		0.50±0.58	0.00±0.00	1.25±0.96	0.75±0.96	0.50±0.58	0.25±0.50
PR extract	0.5	0.25±0.50	0.00±0.00	1.25±0.50	1.00±0.00	0.50±0.58	0.50±0.58
	5	0.75±0.50	0.00±0.00	1.00±0.82	0.25±0.50	0.25±0.50	0.00±0.00
	50	0.75±0.50	0.00±0.00	1.75±0.50	1.00±0.00	0.50±0.58	0.50±0.58
	500	0.25±0.50	0.00±0.00	0.50±0.58	0.25±0.50	0.25±0.50	0.00±0.00
	1,000	0.50±0.58	0.00±0.00	1.50±1.00	1.00±0.82	0.75±0.50	0.25±0.50
	5,000	0.50±0.58	0.00±0.00	1.00±0.00	0.50±0.58	0.25±0.50	0.25±0.50
Cyclophosphamide	6	26.75±3.30b	23.50±2.38b	28.75±3.50b	25.50±2.65b	0.75±0.50	1.25±0.96
Treatment time 6 h, recovery time 18 h, without S9
Vehicle controla		1.00±0.82	0.25±0.50	2.00±1.41	1.25±0.96	0.50±0.58	0.50±0.58
PR extract	0.5	0.25±0.50	0.00±0.00	1.00±0.82	0.75±0.50	0.25±0.50	0.50±0.58
	5	0.50±0.58	0.00±0.00	1.50±0.58	1.00±0.82	0.50±0.58	0.50±0.58
	50	1.00±0.82	0.25±0.50	1.50±1.29	0.75±0.96	0.00±0.00	0.50±0.58
	500	0.25±0.50	0.00±0.00	0.75±0.50	0.50±0.58	0.25±0.50	0.25±0.50
	1,000	0.75±0.96	0.25±0.50	1.50±1.29	1.00±0.82	0.50±0.58	0.25±0.50
	5,000	0.75±0.96	0.25±0.50	1.25±0.96	0.75±0.50	0.25±0.50	0.25±0.50
Ethylmethanesulfonate	800	38.50±6.14b	32.00±4.97b	39.75±6.55b	33.25±5.38b	0.50±0.58	0.75±0.50
Treatment time 24 h, recovery time 0 h, without S9
Vehicle controla		1.75±1.71	0.75±0.96	2.75±1.71	1.75±0.96	0.25±0.50	0.50±0.58
PR extract	0.5	2.25±1.26	0.75±0.96	3.25±2.22	1.75±1.71	0.00±0.00	0.50±0.58
	5	2.00±1.63	1.00±0.82	3.25±2.22	2.25±1.50	0.50±0.58	0.50±0.58
	50	2.00±1.83	0.75±0.96	3.25±0.96	2.00±0.82	0.50±0.58	0.25±0.50
	500	1.00±0.82	0.25±0.50	1.25±0.50	0.50±0.58	0.25±0.50	0.00±0.00
	1,000	1.25±1.89	0.50±1.00	3.00±2.83	2.25±2.22	0.50±1.00	0.75±0.96
	5,000	1.00±0.82	0.50±1.00	2.00±0.82	1.50±0.58	0.25±0.50	0.25±0.50
Ethylmethanesulfonate	600	56.25±11.41b	48.25±10.21b	59.25±13.57b	51.25±12.50b	0.75±0.50	1.00±0.82

Values are expressed as mean ± standard deviation of 100 metaphase cells from quadruple flasks/dose. PR, Persicariae Rhizoma; PP, polyploid; ER, endoreduplication.

^aVehicle control, sterile distilled water.

^bP<0.05 vs. vehicle control by Mann Whitney U test.