Table V.

Results of micronucleus test.

		Body weight (g)
Treatment	Dose, mg/kg	First treatment	Sacrifice	No. of MNPCEa	PCE/(PCE + NCE)b	Slight diarrhea, n/total	Mortality, n/total
Vehicle controlc		31.73±0.88	36.31±1.75	1.30±1.42	0.55±0.05	0/10	0/10
PR extract	2,000	31.88±1.03	36.35±1.63	0.90±0.88	0.55±0.05	3/10	0/10
	1,000	31.78±1.55	36.08±2.08	1.10±0.99	0.51±0.07	0/10	0/10
	500	31.60±1.17	36.30±1.08	0.40±0.52	0.51±0.05	0/10	0/10
Cyclophosphamide	70	31.92±0.86	36.08±0.97	41.70±16.89e	0.31±0.03d	0/10	0/10

Values are expressed as mean ± standard deviation of 10 mice. PR, Persicariae Rhizoma; MNPCE, micronucleated bone marrow polychromatic erythrocyte; PCE, polychromatic erythrocyte; NCE, normochromatic erythrocyte. Animals were treated for 2 consecutive days (vehicle and PR extract) or once (cyclophosphamide) and sacrificed 24 h after the last dose.

^a≥2,000 PCEs per animal were analyzed to determine the frequency of micronuclei.

^bCytotoxicity was assessed by scoring the number of PCEs and NCEs in at least the first 500 erythrocytes for each animal.

^cVehicle control, sterile distilled water.

^dP<0.01 vs. vehicle control by Scheffe's test.

^eP<0.01 vs. vehicle control by Mann Whitney U test.