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. 2016 Jun 14;2:16013. doi: 10.1038/celldisc.2016.13

Figure 3.

Figure 3

Relationship between intronic APA and gene expression regulation. (a) Gene expression changes vs intron-APA regulation. The blue line is for genes with activated intronic pA in 2 vs 0.5 h (RED<−1), and green line for other genes. X-axis indicates log2 ratio of gene expression in 2 vs 0.5 h in RKO (left) and RKO-E6 (right). Y-axis is cumulative fraction. The median value of each group is indicated by a dotted vertical line. The P-value is based on comparison of two groups using the Kolmogorov–Smirnov test. (b) As in a except that gene expression changes are based on 0.5 vs 0 h. Note that intronic regulation is based on 2 vs 0.5 h (RED<−1) as in a. (c, d) Significant functional categories enriched with genes with significant intronic APA regulation in both RKO and RKO-E6 cells (underlined in the Venn diagram in Figure 2e, right panel), as analyzed by the Ingenuity Pathway Analysis Network (c) and Molecular and Cellular Function terms (d). In the network, red nodes indicate genes with significant intronic APA regulation. APA, alternative cleavage and polyadenylation.