Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 14;2:16013. doi: 10.1038/celldisc.2016.13

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 SIBS, CAS

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Effect of UV treatment on APA. (a) Schematic representation of amplified regions to detect 3ʹUTR and intronic APA. HCT116 were treated with UV irradiation and allowed to recover for indicated times, and then harvested. cDNA was prepared using oligo(dT) primers from nuclear RNA and used for PCR and qPCR reactions with primers specific for intronic APA, full-length mRNA, and 3ʹUTR (proximal and distal) APA. (b) qRT–PCR analysis of HCT116 samples for the effect of UV on 3ʹUTR pA choice. The ratio of distal/proximal of each analyzed gene is shown. Samples were prepared as described in a. The qRT–PCR values were calculated from three independent biological samples by triplicate. (c) Intron-APA is transiently upregulated on UV-induced DNA damage. Samples were analyzed as in a but the ratio of intronic/full-length of each mRNA is shown. The qRT–PCR values were calculated from three independent samples. APA, alternative cleavage and polyadenylation; cDNA, complementary DNA; qRT–PCR, quantitative reverse transcription–PCR; UV, ultraviolet.