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. 2016 Jun 13;9:124. doi: 10.1186/s13068-016-0544-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Enzymatic properties of wild-type TlGlu16A and its mutants. a pH-dependent activity profiles. b pH stability. c Temperature-dependent activity profiles. d Enzyme inactivation at different temperatures for 30 min