Table 1.

Half-lives of wild-type TlGlu16A and its mutants for thermal inactivation

Enzyme	t 1/2 (min) at
	65 °C	70 °C	75 °C	80 °C
TlGlu16A	25	3	1	0.5
H58D	77	56	37	31
E134R	41	3	4.5	7
D235G	39	4	3.5	25
D296K	60	15	30	22

The enzyme activity was assayed at each optimal condition for 10 min