Table 3.

Specific activities and kinetics of wild-type TlGlu16A and its mutants with barley β-glucan and lichenan as the substrate

Enzymes	Barley β-glucan				Lichenan
	Specific activity (U/mg)	V max (μmol/min·mg)	V max (μmol/min·mg)	k cat/K m (mL/s·mg)	Specific activity (U/mg)	K m (mg/mL)	K m (mg/mL)	K cat/K m (mL/s·mg)
TlGlu16A	15,197 ± 153	5.74 ± 0.63	38,314 ± 187	3944 ± 156	12,770 ± 98	3.69 ± 0.54	13,447 ± 112	3643 ± 115
H58D	15,066 ± 113	2.52 ± 0.75	22,936 ± 113	5384 ± 127	13,408 ± 87	3.45 ± 0.46	27,100 ± 198	4637 ± 99
E134R	11,996 ± 79	5.04 ± 0.76	26,810 ± 154	3144 ± 143	7715 ± 88	2.64 ± 0.93	13,351 ± 167	2988 ± 198
D235G	24,040 ± 102	5.41 ± 1.01	61,350 ± 187	6701 ± 154	13,886 ± 102	9.32 ± 0.98	50,761 ± 201	3221 ± 154
D296K	18,574 ± 103	3.35 ± 0.43	25,381 ± 201	4479 ± 163	14,488 ± 101	7.39 ± 1.41	22,669 ± 175	3067 ± 103

Values are means ± standard deviations