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Immunohistochemistry with F4/80 antibody (×200 original magnification) at 4 weeks: a standard diet (MF) group; b high-fat, choline-deficient diet (HFCD) + diethylnitrosamine (DEN) group; c the ratio of F4/80-positive cells (macrophages) to hepatocyte nuclei; d serum amyloid A (SAA) immunostaining; e representative images of liver sections stained with Sirius red at 24 weeks; and f proportion of fibrotic area measured using Histoquest. The data are shown as the mean + standard deviation
