Table 4.

Growth conditions of MG1655 and C58

Name	Conditions	Harvest point
E. coli MG1655
Standard	Grown at 37 °C in LB media	Reached mid-log phase O.D. 600 nm 0.6 ~ 0.8
Cold shock	Grown at 15 °C for 4 h then grown at 37 °C in LB media	Reached half of O.D. 600 nm of Standard
Heat shock	Grown at 50 °C for 4 h then grown at 37 °C in LB media	Same as above
Low pH	Grown at 37 °C for 1 h in LB media with pH4.5 then grown at 37 °C in LB media	Same as above
UV treat	Exposed to UV light for 15 min then grown at 37 °C in LB media	Same as above
Low carbon	Grown at 37 °C in minimal C source MOPS media [49] (C- MOPS)	Same as above
Low nitrogen	Grown at 37 °C in minimal N source MOPS media (N- MOPS)	Same as above
Low C & N	Grown at 37 °C in minimal C and N source MOPS media (C-N- MOPS)	Reached quarter of O.D. 600 nm of Standard
Oxidative	Growth at 37 °C in 49 mL MOPS media with 400 μL 7 % Hydrogen peroxide	Reached half of O.D. 600 nm of Standard
Osmotic	Growth at 37 °C in 45 mL MOPS media with 6 mL 4 M Sodium Chloride	Same as above
A. tumefaciens C58
Standard	Grown at 28 °C in YEM media	Reached mid-log phase O.D. 600 nm 0.6 ~ 0.8
Cold Shock	Grown at 17 °C for 13 h then grown at 28 °C in YEM media	Reached half of O.D. 600 nm of Standard
Heat shock	Grown at 40 °C for 11 h then grown at 28 °C in YEM media	Same as above
Low pH	Grown at 28 °C in AB5.5 media [50]	Reached mid-log phase
Low Iron	Grown at 28 °C in AB7 without Fe media (AB7 Fe-) [51]	Same as above
Oxidative	Grown at 28 °C in 40 mL YEM media with 130 μL 1 % Hydrogen peroxide	Reached half of O.D. 600 nm of Standard
Cold shock & oxidative	Grown at 17 °C for 13 h then grown at 28 °C in YEM media	Reached quarter of O.D. 600 nm of Standard
Low pH & cold shock	Grown at 17 °C for 13 h then grown at 28 °C in AB5.5 media	Same as above
Low pH & heat shock	Grown at 40 °C for 11 h then grown at 28 °C in AB5.5 media	Same as above
Low pH & low Iron	Grown at 28 °C in AB5.5 without Fe media (AB5.5 Fe-)	Same as above