Table 1.
Monoclonal antibodies used for the characterization of stem cell markers and cell phenotypes
| Primary antibody/localization marker | Code number | Dilution | Incubation |
|---|---|---|---|
| HLA-DR, surface | Santa Cruz, sc-18875 | 1:50 | o/n, r.t. |
| CD34, surface | Santa Cruz, sc-19621 | 1:50 | o/n, r.t. |
| CD45, surface | Santa Cruz, sc-28369 | 1:50 | o/n, r.t. |
| SSEA4, surface | Miltenyi Biotec, 130-98-371 | 1:100 | 30 minutes, r.t. |
| ABCG2, surface | BioLegend, 332002 | 1:100 | o/n, r.t. |
| Δnp63, nuclear | BioLegend, 619002 | 1:100 | o/n, r.t. |
| NANOG, surface | Santa Cruz, sc-293121 | 1:50 | o/n, r.t. |
| OCT4, nuclear | Santa Cruz, sc-5279 | 1:50 | o/n, r.t. |
| Secondary antibody | Code number | Dilution | Incubation |
| AlexaFluor 488 | Life Technologies, Z25402 | 1:50 | 20 minutes, r.t. |
| AlexaFluor 594 | Life Technologies, Z25007 | 1:50 | 20 minutes, r.t. |
Miltenyi Biotec, Bergisch Gladbach, Germany
Santa Cruz, Dallas, Texas
BioLegend, London, UK
Life Technologies, Carlsbad, CA, USA
o/n overnight, r.t. room temperature