Fig. 5.

Immunolocalization of C. sinensis GSTo1 and compartmental expression of C. sinensis GSTo1 and 2 transcripts. a Adult worm sections (thickness 4-μm) were incubated with preimmune mouse serum (panels 1) or anti-rCsGST1 antibody (panels 2) at a same dilution ratio (1:200) and subsequently incubated with HRP-conjugated goat anti-mouse IgG antibody (1:1,000 dilutions). The slides were developed with the blue-immunohistochemistry chromogen 3,3'-diaminobenzidine blue supplemented with H₂O₂. Acetocarmine-stained in toto specimen is also seen. The regions marked by dotted-ellipses were separately prepared as vitelline follicle-enriched parenchyma for Western blot (see also Fig. 7). Abbreviations: OS, oral sucker; IN, intestine; VS, ventral sucker; VF, vitelline follicles; SR, seminal receptacle; TE, testis. Scale-bars: 50 μm. b Expression of CsGSTo transcripts in C. sinensis reproductive system by qRT-PCR. Total RNAs (each 200 ng) extracted from respective organs were reverse transcribed into cDNA and subjected to qRT-PCR. The fold increase was calculated by differences in threshold cycles (ΔΔC_T). Key: 1, egg; 2, whole worm; 3, testis, seminal receptacle and vitelline follicle-enriched fractions; 4, parenchymal fractions without reproductive organs