Table 1.
Substrate specificity of recombinant CsGSTo1 and 2
| Substrate | Class-specificity | Specific activity (μmol/min/mg) | ||
|---|---|---|---|---|
| rGSTo1 | rGSTo2 | |||
| GST-specific | CDNBa | All | 0.13 ± 0.01 | 0.10 ± 0.02 |
| Cumene hydroperoxide | α | ndb | nd | |
| DCNBc | μ | nd | nd | |
| Ethacrynic acid | π | nd | nd | |
| 4-nitrobenzyl chloride | μ and θ | 1.42 ± 0.09 | 0.91 ± 0.24 | |
| 4-nitrophenyl acetate | ω | 0.84 ± 0.06 | 0.73 ± 0.04 | |
| 4-hydroxy nonenal | α and θ | nd | nd | |
| DHARd | Dehydroascorbate | ω | 1.16 ± 0.04 | 1.08 ± 0.02 |
| Thioltransferase | Hydroxyethyl disulfide | ω | 1.08 ± 0.02 | 0.76 ± 0.02 |
a1-chloro-2,4-dinitrobenzene
bNot detected
c1,2-dichloro-4-nitrobenzene
dDehydroascorbate reductase