Fig. 5.

Representative time series of internalization of fluorescently labeled (AF568) anti-DEspR 7c5b2-mAb by Panc1 tumor cells within 1.5 h. a Confocal images showing representative Panc1 tumor cells from baseline (t-0) prior to addition of AF568-labeled antibody, up to 1 h, 15 min from addition of AF568-7c5b2 mAb. Increasing intracellular fluorescence (white) is detected in multiple Panc1 cells. b Higher magnification of Panc1 tumor cells at baseline and t-75 min with corresponding bright field images of Panc1 cells. At t-60 min, representative image of Panc1 tumor cells exposed to control AF568-labeled IgG2b isotype, with DAPI stained nuclei (blue) to mark cells, demonstrate no intracellular AF568 fluorescence (red) uptake. Bar = 20 microns