Table 1.
Proteins pulled-down with anti-DEspR mAb 5g12e8 from U87 CSC membranes
| Accession | % | #SM | #P | #AAs | Mass | Score | Description |
|---|---|---|---|---|---|---|---|
| P08670 | 55.8 | 36 | 31 | 466 | 53,619 | 177.59 | Vimentin (VIM) |
| P60709 | 34.4 | 11 | 9 | 375 | 41,709 | 66.79 | Actin, cytoplasmic 1 (ACTB) |
| P68371 | 41.6 | 13 | 12 | 445 | 49,799 | 56.53 | Tubulin beta-4B chain (TUBB4B) |
| P07437 | 35.1 | 11 | 10 | 444 | 49,638 | 47.84 | Tubulin beta chain (TUBB) |
| Q13885 | 29.0 | 10 | 9 | 445 | 49,874 | 42.11 | Tubulin beta-2A chain (TUBB2A) |
| P17931 | 25.6 | 7 | 5 | 250 | 26,136 | 39.26 | Galectin-3 (LGALS3) |
| P09382 | 49.6 | 7 | 6 | 135 | 14,706 | 38.90 | Galectin-1 (LGALS1) a |
| Q13509 | 25.6 | 8 | 8 | 450 | 50,400 | 32.86 | Tubulin beta-3 chain (TUBB3) |
| Q9BUF5 | 15.7 | 6 | 5 | 446 | 49,825 | 21.46 | Tubulin beta-6 chain (TUBB6) |
| P61006 | 13.0 | 2 | 2 | 207 | 23,653 | 12.01 | Ras-related protein Rab-8A (RAB8A) |
| C9J8S3 | 15.0 | 2 | 2 | 160 | 18,015 | 11.01 | Ras-related protein Rab-7a (RAB7A) |
| P61026 | 6.0 | 1 | 1 | 200 | 22,526 | 9.59 | Ras-related protein Rab-10 (RAB10) |
| P51153 | 5.9 | 1 | 1 | 203 | 22,759 | 7.60 | Ras-related protein Rab-13 (RAB13) |
| H0YNE9 | 6.4 | 1 | 1 | 188 | 21,854 | 7.51 | Ras-related protein Rab-8B (Fragment) (RAB8B) |
| G3V2K7 | 7.2 | 1 | 1 | 153 | 16,893 | 5.96 | Transmembrane emp24 domain-containing protein 10 (TMED10) a |
| Q9Y3B3-2 | 5.3 | 1 | 1 | 188 | 21,219 | 4.85 | Transmembrane emp24 domain-containing protein 7 (TMED7) |
| Q9BVK6 | 3.8 | 1 | 1 | 235 | 27,260 | 4.36 | Transmembrane emp24 domain-containing protein 9 (TMED9) |
| F8WBC0 | 34.4 | 1 | 1 | 32 | 3501 | 2.90 | Ras-related protein Rap-1b (Fragment) (RAP1B) |
Proteins in italic face letters are proteins detected in three independent pull-down experiments
% the percentage of the protein sequence covered by identified peptides; #SM the number of peptide spectrum matches; #P the total number of distinct peptide sequences identified in the protein group; #AAs number of amino acids; Mass, mass in Daltons; Score, the sum of the ion scores of all peptides that were identified
aProteins verified by Western blot analysis of pull-down proteins; Rab-1b was detected in two independent experiments and verified by Western blot analysis (Fig. 2b)