Table 3.
Overexpression of single common stress-responsive miRNA for conferring abiotic stress tolerance in model and crop plants.
| miRNA | Source of the targeted miRNA gene | Target | Transgenic plant | Expression strategy | Response | References |
|---|---|---|---|---|---|---|
| miR156 | Oryza sativa | SPL | Oryza sativa | Overexpression of OsmiR156k | Reduced cold tolerance | Cui et al., 2015 |
| miR172 | Glycine max | AP2 like TFs | Arabidopsis | Overexpression of gma-miR172c | Enhanced Water deficit and salt tolerance | Li et al., 2016 |
| miR319 | O. sativa | PCF5 and PCF8 | Oryza sativa | RNAi | Enhanced cold tolerance | Yang C. et al., 2013 |
| miR319 | O. sativa | TCP | Agrostis stolonifera | Constitutive overexpression of osa-miR319a | Enhanced drought and salt tolerance | Zhou et al., 2013 |
| miR390 | O. sativa | SRK | Oryza sativa | Overexpression of miR390 | Reduced Cd tolerance/enhanced Cd accumulation | Ding et al., 2016 |
| miR394a | G. max | F-box Protein | Arabidopsis | Overexpression of gma-miR394a | Enhanced drought tolerance | Ni et al., 2012 |
| miR394a | Arabidopsis thaliana | LCR | Arabidopsis | Overexpression of miR394a/LCR loss of function mutant | Enhanced cold tolerance | Song et al., 2016 |
| miR395 | A. thaliana | BnSultr, BnAPS | Brassica napus | Overexpression of miR395 driven by CaMV35S promoter | Shorten or no surface trichomes with delayed transition from juvenile to adult vegetative stage | Huang et al., 2010 |
| miR398 | A. thaliana | CSD1, CSD2, CCS | Arabidopsis | Loss of function mutants of CSD1 and CCS, knockdown mutant of CSD2 | Enhanced thermo tolerance | Guan et al., 2013 |
| miR399 | A. thaliana | IPS-1 | Solanum lycopersicum | overexpression of Ath-miR399d under control of rd29A promoter | Better growth performances under phosphorus deficiency and low temperature | Gao et al., 2015 |
| miR408 | A. thaliana | Copper related gene | Cicer arietinum | Overexpression of Athpre-miR408 | Enhanced drought tolerance | Hajyzadeh et al., 2015 |
SPL, Squamosa promoter binding protein-like; AP, Apetala; TFs, Transcription factors; TCP, Teosinte Branched Cycloidea and PCF family; PCF, Proliferating cell factors; SRK, Stress responsive leucine rich repeat receptor like kinases; LCR, Leaf curling responsiveness; CSD, Copper/Zinc superoxide dismutase; CCS, Copper Chaperon of CSD; IPS, IFN-β Promoter stimulator; BnSultr, Brassica napus sulfate transporters; BnAPS, B. napus ATP sulfurylases.