Table 1.

Relationship Between Chronological Age, Smoking, and Alcohol Consumption Biomarkers and Aging Markers in the Hannum Cohort

Aging Related Markers	Chronological Age		cg05575921		cg23193759
	Beta	P-Value	Beta	P-Value	Beta	P-Value
cg16867657	0.859	<.001	−0.026	.20	0.038	.06
cg06639320	0.747	<.001	0.019	.47	0.161	<.001
cg22454769	0.744	<.001	0.028	.28	0.092	<.001
cg24079702	0.707	<.001	0.023	.41	0.088	.002
cg07553761	0.700	<.001	−0.059	.03	−0.044	.12
cg04875128	0.665	<.001	−0.019	.51	0.119	<.001
cg14692377	0.654	<.001	0.054	.07	0.120	<.001
cg22736354	0.645	<.001	0.021	.47	0.171	<.001
cg07547549	0.631	<.001	0.018	.56	0.203	<.001
cg02650266	0.628	<.001	−0.076	.01	0.172	<.001
cg23500537	0.620	<.001	−0.029	.34	0.064	.04
cg03032497	0.620	<.001	−0.053	.08	0.036	.24
cg08097417	0.615	<.001	0.087	.005	0.119	<.001
cg14361627	0.614	<.001	0.064	.04	0.072	.02
cg16419235	0.610	<.001	−0.015	.63	0.187	<.001
cg22285878	0.592	<.001	−0.012	.71	0.020	.54
cg03607117	0.586	<.001	0.028	.38	0.012	.71
cg06493994	0.585	<.001	0.088	.006	0.278	<.001
cg04400972	0.577	<.001	−0.058	.07	0.133	<.001
cg23091758	0.559	<.001	−0.010	.75	0.103	.002
cg07955995	0.553	<.001	0.053	.10	0.009	.79
cg22158769	0.548	<.001	−0.056	.08	0.100	.002
cg20426994	0.544	<.001	−0.003	.94	0.150	<.001
cg14556683	0.538	<.001	0.033	.32	0.229	<.001
cg00748589	0.534	<.001	0.051	.12	0.300	<.001
cg21296230	0.533	<.001	0.029	.38	0.203	<.001
cg07927379	0.529	<.001	0.036	.27	0.044	.19
cg25410668	0.523	<.001	−0.020	.54	0.141	<.001
cg22213242	0.513	<.001	0.050	.13	−0.110	.001
cg23606718	0.512	<.001	0.037	.27	0.290	<.001
cg03399905	0.509	<.001	−0.012	.72	0.308	<.001
cg25478614	0.506	<.001	0.054	.11	0.078	.02
cg06419846	0.504	<.001	−0.005	.89	−0.093	.007
cg00481951	0.495	<.001	0.022	.52	0.211	<.001
cg08540945	0.491	<.001	−0.026	.45	0.153	<.001
cg18473521	0.477	<.001	0.001	.97	0.049	.16
cg11067179	0.462	<.001	0.006	.86	−0.123	<.001
cg04940570	0.461	<.001	0.024	.49	0.278	<.001
cg21139312	0.377	<.001	0.061	.09	−0.001	.97
cg19935065	0.237	<.001	0.022	.57	0.023	.55
ch1339564907R	−0.372	<.001	0.028	.43	0.001	.98
cg09651136	−0.401	<.001	0.084	.02	0.093	.01
ch230415474F	−0.413	<.001	0.072	.04	0.001	.97
cg13001142	−0.416	<.001	−0.017	.63	0.124	.001
cg05442902	−0.439	<.001	0.147	<.001	0.112	.002
cg02867102	−0.474	<.001	0.116	.001	0.140	<.001
cg00486113	−0.521	<.001	0.204	<.001	0.194	<.001
cg20052760	−0.526	<.001	0.111	.001	0.212	<.001
cg19722847	−0.534	<.001	0.125	<.001	0.189	<.001
cg06874016	−0.537	<.001	0.109	.001	0.033	.33
cg02046143	−0.538	<.001	0.095	.004	0.241	<.001
cg25428494	−0.544	<.001	0.080	.01	0.133	<.001
cg04474832	−0.548	<.001	0.188	<.001	0.191	<.001
cg02085953	−0.556	<.001	0.159	<.001	0.037	.26
cg04416734	−0.557	<.001	0.205	<.001	0.120	<.001
cg22512670	−0.565	<.001	0.206	<.001	0.201	<.001
cg06685111	−0.568	<.001	0.162	<.001	0.213	<.001
cg03473532	−0.569	<.001	0.100	.002	0.133	<.001
cg22016779	−0.572	<.001	0.036	.27	−0.009	.77
cg20822990	−0.576	<.001	0.191	<.001	0.293	<.001
cg08415592	−0.578	<.001	0.109	.001	0.145	<.001
cg07583137	−0.589	<.001	0.146	<.001	0.121	<.001
cg09809672	−0.598	<.001	0.162	<.001	0.226	<.001
cg01528542	−0.604	<.001	0.064	.04	−0.005	.88
cg07082267	−0.608	<.001	0.186	<.001	0.239	<.001
cg22796704	−0.616	<.001	0.157	<.001	0.229	<.001
cg23744638	−0.640	<.001	0.150	<.001	0.196	<.001
cg16054275	−0.646	<.001	0.124	<.001	0.093	.002
cg08234504	−0.669	<.001	0.125	<.001	0.153	<.001
cg19283806	−0.679	<.001	0.068	.02	0.125	<.001
cg10501210	−0.726	<.001	0.108	<.001	0.152	<.001

Note. The loci in the top half of the table are those that hypermethylate (+beta) and those in the bottom half are those that demethylate (−beta) as a function of increasing age. Significant pro-aging effects of smoking or alcohol consumption as denoted by cg05575921 or cg23193759, respectively, are indicated in bold. Antiaging effects of cigarette or alcohol consumption are denoted by bold italics