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. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: J Am Chem Soc. 2016 Mar 11;138(11):3789–3796. doi: 10.1021/jacs.5b13233

Figure 2.

Figure 2

Long-term stability of LeuT solubilized in DDM or a novel detergent (PSA-C10, PSA-C11, PSE-C9, PSE-C11, or PSE-C13) at concentrations of CMC + 0.04 wt% (a) and CMC + 0.2 wt% (b). DDM-purified transporter was mixed with individual detergent-containing solutions and then incubated for 12 days at room temperature. LeuT activity was measured at regular intervals during the incubation, by radiolabeled ligand ([3H]-Leu) binding via scintillation proximity assay (SPA).