Figure 4.

Chrysin regulates the p53/Bcl-2/caspase-9 signaling pathway. Chrysin regulates the content of apoptotic-related proteins in HepG2 and QGY7701 cells following various concentrations of chrysin treatment for 24 h. (A) Western blotting was performed in order to analyze the protein content of p53, Bcl-2, Bax, Bak, Bad, caspases-3 and −9, and cleaved-caspases-3 and −9 (B) and this data was subsequently quantified. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 µg/ml chrysin. Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X; Bad, Bcl-2-associated death promoter; Bak, Bcl-2 homologous antagonist/killer.