Table I.
Oligonucleotide primers for reverse transcription-quantitative polymerase chain reaction analysis.
| Gene | Genbank accession no. | Primer sequence |
|---|---|---|
| ZnT-2 | RNU50927 | F, 5′-GGCTGGATCCTGGACTAATGTT-3′ |
| R, 5′-ACACCCCAAAATCCCTTTCTG-3′ | ||
| Probe, 5′-FAM-CTCACACCACAGCTGGAGAGACACTGAGG-TAMRA-3′ | ||
| ZnT-4 | NM_172066.1 | F, 5′-GCTGAAGCAGAGGAAGGTGAA-3′ |
| R, 5′-TCTCCGATCATGAAAAGCAAGTAG-3′ | ||
| Probe, 5′-FAM-CAGGCTGACCATCGCTGCCGT-TAMRA-3′ | ||
| ZnT-5 | NM_001106404.1 | F, 5′-CCAGCACATGTCTGGCCTAA-3′ |
| R, 5′-TTTGCAGTACTTCATGGATTCCA-3′ | ||
| Probe,5′-FAM-CACTGGCTTCCACGATGTCCTGGCTAT-TAMRA-3′ | ||
| ZnT-6 | NM_001106708.1 | F, 5′-CGGCATTATCCCAGGACTCA-3′ |
| R, 5′-CCAGCAAGATCGATCAGAACAA-3′ | ||
| Probe, 5′-FAM-TTCTTGCCCCGCATGAACCCG-TAMRA-3′ | ||
| ZnT-7 | XM_001073594.1 | F, 5′-TTGGGATCCGCGTCTGA-3′ |
| R, 5′-CCCTCTAGAAGTGACTCGGTATGG-3′ | ||
| Probe, 5′-FAM-TCGTCTCTGCTGTCACTGCCGCC-TAMRA-3′ | ||
| DCT-1 | NM_001108855.1 | F, 5′-TGGCTGCCTCAGCATCTG-3′ |
| R, 5′-CCCAGTTGTTGAGTGGTTTGG-3′ | ||
| Probe, 5′-FAM-AAAACCAGAGCCCCTTCCCTACGCA-TAMRA-3′ | ||
| ZIP-6 | NM_001024745.1 | F, 5′-CTCACTGCTGGCTTGTTCATG-3′ |
| R, 5′-CGTGATCACTGGCATCATTGT-3′ | ||
| Probe, 5′-FAM-TCGCTCTGGTCGACATGGTACCCG-TAMRA-3′ | ||
| ZIP-7 | NM_001008885.1 | F, 5′-GACTTGGCACACAACTTCACAGA-3′ |
| R, 5′-GGACTGTCATCGTGGTCAGGAT-3′ | ||
| Probe, 5′-FAM-CTGGCCATTGGTGCTTCCTTCCG-TAMRA-3′ | ||
| Cathepsin D | NM_134334 | F, 5′-CATCGCAGCCAAGTTTGATG-3′ |
| R, 5′-CCGGGAGCACATTGTTAACA-3′ | ||
| Probe, 5′-FAM-CATCTTGGGCATGGGCTACCCTTTTATC-TAMRA-3′ | ||
| Cathepsin L | NM_013156 | F, 5′-GGGTATGGATGGCTACATCAAAA-3′ |
| R, 5′-GGCTGCGGTGGCAAGTC-3′ | ||
| Probe, 5′-FAM-AGCCAAAGACCGGAACAACCACTGC-TAMRA-3′ |
ZnT, zinc transporter; DCT-1, divalent cation transporter 1; ZIP, Zrt-Irt-like protein.