Table 3.
Genotype | Ia | Ib | II | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PRV isolate 2 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | Ia |
KT456503 | - | 99.9 | 99.8 | 99.8 | 99.6 | 99.6 | 99.6 | 99.6 | 99.6 | 99.5 | 99.3 | 99.3 | 99.2 | 99.0 | 98.7 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 95.9 | 95.8 | 80.7 | 80.5 | nd | |
KU131593 a | 100 | - | 99.6 | 99.6 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.4 | 99.2 | 99.2 | 99.0 | 98.9 | 98.6 | 95.9 | 95.9 | 95.9 | 95.9 | 95.9 | 95.9 | 95.8 | 95.7 | 80.9 | 80.3 | nd | |
KU131594 a | 100 | 100 | - | 99.5 | 99.6 | 99.4 | 99.4 | 99.4 | 99.4 | 99.3 | 99.0 | 99.0 | 98.9 | 98.9 | 98.4 | 95.8 | 95.8 | 95.8 | 95.8 | 95.8 | 95.8 | 95.7 | 95.5 | 80.6 | 80.4 | nd | |
KC473454 | 99.3 | 99.3 | 99.3 | - | 99.4 | 99.4 | 99.4 | 99.4 | 99.4 | 99.3 | 99.0 | 99.0 | 98.9 | 98.8 | 98.4 | 95.8 | 95.8 | 95.8 | 95.8 | 95.8 | 95.8 | 95.7 | 95.5 | 80.5 | 80.3 | nd | |
KU131591 a | 100 | 100 | 100 | 99.3 | - | 99.3 | 99.3 | 99.3 | 99.3 | 99.2 | 98.9 | 98.9 | 98.8 | 98.8 | 98.3 | 95.7 | 95.7 | 95.7 | 95.7 | 95.7 | 95.7 | 95.5 | 95.4 | 80.7 | 80.5 | nd | |
KR872636 | 99.6 | 99.6 | 99.6 | 98.9 | 99.6 | - | 99.3 | 99.5 | 99.5 | 99.6 | 99.4 | 99.4 | 99.0 | 99.2 | 98.6 | 95.9 | 95.9 | 95.9 | 95.9 | 95.9 | 95.9 | 95.8 | 95.7 | 80.6 | 80.4 | nd | |
KT456500b | 98.9 | 98.9 | 98.9 | 98.2 | 98.9 | 98.6 | - | 99.3 | 99.3 | 99.2 | 98.9 | 99.2 | 98.8 | 98.7 | 98.3 | 95.7 | 95.7 | 95.7 | 95.7 | 95.7 | 95.7 | 95.5 | 95.4 | 80.4 | 80.2 | nd | |
JN991006 | 100 | 100 | 100 | 99.3 | 100 | 99.6 | 98.9 | - | 99.8 | 99.4 | 99.2 | 99.2 | 99.3 | 98.9 | 98.8 | 96.3 | 96.3 | 96.3 | 96.3 | 96.3 | 96.3 | 96.1 | 96.0 | 80.6 | 80.4 | nd | |
HG329868 | 100 | 100 | 100 | 99.3 | 100 | 99.6 | 98.9 | 100 | - | 99.4 | 99.2 | 99.2 | 99.5 | 98.9 | 99.0 | 96.4 | 96.4 | 96.4 | 96.4 | 96.4 | 96.4 | 96.3 | 96.1 | 80.9 | 80.6 | nd | |
KU160514 | 99.6 | 99.6 | 99.6 | 98.9 | 99.6 | 99.3 | 98.6 | 99.6 | 99.6 | - | 99.3 | 99.5 | 98.9 | 99.3 | 98.4 | 95.8 | 95.8 | 95.8 | 95.8 | 95.8 | 95.8 | 95.7 | 95.5 | 80.2 | 80.3 | nd | |
KT456501 | 98.6 | 98.6 | 98.6 | 97.8 | 98.6 | 98.2 | 97.5 | 98.6 | 98.6 | 98.2 | - | 99.0 | 98.7 | 98.9 | 98.2 | 95.5 | 95.5 | 95.5 | 95.5 | 95.5 | 95.5 | 95.4 | 95.3 | 80.5 | 80.3 | nd | |
KC473453 | 99.6 | 99.6 | 99.6 | 98.9 | 99.6 | 99.3 | 99.3 | 99.6 | 99.6 | 99.3 | 99.3 | - | 98.7 | 99.0 | 98.2 | 95.5 | 95.5 | 95.5 | 95.5 | 95.5 | 95.5 | 95.4 | 95.3 | 80.3 | 80.0 | nd | |
KT456505 | 98.6 | 98.6 | 98.6 | 97.8 | 98.6 | 98.2 | 97.5 | 98.6 | 98.6 | 98.2 | 97.1 | 98.2 | - | 98.4 | 98.6 | 96.1 | 96.1 | 96.1 | 96.1 | 96.1 | 96.1 | 96.0 | 95.9 | 80.9 | 80.7 | nd | |
KU160513 | 98.9 | 98.9 | 98.9 | 98.2 | 98.9 | 98.6 | 97.8 | 98.9 | 98.9 | 98.6 | 97.5 | 98.6 | 97.5 | - | 98.0 | 95.3 | 95.3 | 95.3 | 95.3 | 95.3 | 95.3 | 95.2 | 95.1 | 80.2 | 79.9 | nd | |
HG329848 | 100 | 100 | 100 | 99.3 | 100 | 99.6 | 98.9 | 100 | 100 | 99.6 | 98.6 | 99.6 | 98.6 | 98.9 | - | 96.4 | 96.4 | 96.4 | 96.4 | 96.4 | 96.4 | 96.3 | 96.1 | 80.9 | 80.7 | nd | |
KU131597 | 96.4 | 96.4 | 96.4 | 95.7 | 96.4 | 96.0 | 95.3 | 96.4 | 96.4 | 96.0 | 95.3 | 96.0 | 95.3 | 95.3 | 96.4 | - | 100 | 100 | 100 | 100 | 100 | 99.9 | 99.8 | 79.2 | 79.0 | nd | Ib |
HG329897c | 96.4 | 96.4 | 96.4 | 95.7 | 96.4 | 96.0 | 95.3 | 96.4 | 96.4 | 96.0 | 95.3 | 96.0 | 95.3 | 95.3 | 96.4 | 100 | - | 100 | 100 | 100 | 100 | 99.9 | 99.8 | 79.2 | 79.0 | nd | |
KU131602 | 96.4 | 96.4 | 96.4 | 95.7 | 96.4 | 96.0 | 95.3 | 96.4 | 96.4 | 96.0 | 95.3 | 96.0 | 95.3 | 95.3 | 96.4 | 100 | 100 | - | 100 | 100 | 100 | 99.9 | 99.8 | 79.2 | 79.0 | nd | |
KC795571 | 96.4 | 96.4 | 96.4 | 95.7 | 96.4 | 96.0 | 95.3 | 96.4 | 96.4 | 96.0 | 95.3 | 96.0 | 95.3 | 95.3 | 96.4 | 100 | 100 | 100 | - | 100 | 100 | 99.9 | 99.8 | 79.2 | 79.0 | nd | |
KU131604 a | 96.4 | 96.4 | 96.4 | 95.7 | 96.4 | 96.0 | 95.3 | 96.4 | 96.4 | 96.0 | 95.3 | 96.0 | 95.3 | 95.3 | 96.4 | 100 | 100 | 100 | 100 | - | 100 | 99.9 | 99.8 | 79.2 | 79.0 | nd | |
GU994022 | 96.4 | 96.4 | 96.4 | 95.7 | 96.4 | 96.0 | 95.3 | 96.4 | 96.4 | 96.0 | 95.3 | 96.0 | 95.3 | 95.3 | 96.4 | 100 | 100 | 100 | 100 | 100 | - | 99.9 | 99.8 | 79.2 | 79.0 | nd | |
KU131603 | 96.0 | 96.0 | 96.0 | 95.3 | 96.0 | 95.7 | 94.9 | 96.0 | 96.0 | 95.7 | 94.9 | 95.7 | 94.9 | 94.9 | 96.0 | 99.6 | 99.6 | 99.6 | 99.6 | 99.6 | 99.6 | - | 99.6 | 79.1 | 78.8 | nd | |
KU131598 a | 95.7 | 95.7 | 95.7 | 94.9 | 95.7 | 95.3 | 94.6 | 95.7 | 95.7 | 95.3 | 94.6 | 95.3 | 94.6 | 94.6 | 95.7 | 99.3 | 99.3 | 99.3 | 99.3 | 99.3 | 99.3 | 98.9 | - | 79.0 | 78.7 | nd | |
KU131595 a | 79.0 | 79.0 | 79.0 | 78.3 | 79.0 | 79.0 | 78.3 | 79.0 | 79.0 | 79.0 | 78.3 | 79.0 | 79.0 | 78.3 | 79.0 | 78.3 | 78.3 | 78.3 | 78.3 | 78.3 | 78.3 | 77.9 | 77.5 | - | 99.5 | nd | II |
KU131596 a | 78.6 | 78.6 | 78.6 | 77.9 | 78.6 | 78.6 | 77.9 | 78.6 | 78.6 | 78.6 | 77.9 | 78.6 | 78.6 | 77.9 | 78.6 | 77.9 | 77.9 | 77.9 | 77.9 | 77.9 | 77.9 | 77.5 | 77.2 | 99.3 | - | nd | |
LN680851d | nd | nd | Nd | nd | nd | Nd | nd | nd | nd | Nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | - |
1Pairwise sequence comparision was done with EMBOSS Water on-line program using default settings [52]; values above the diagonal are nucleotide sequence identities (%) in 830 nt overlap; values below the diagonal are deduced amino acid sequence identities of Outer clamp (σ3) protein (%) in 276 aa overlap. Bold text denotes new Chilean PRV sequences generated in this study
2PRV isolates identified in vertical column by GenBank Accession Numbers and on the top horizontal row by order of listing in the vertical column
3nd denotes not done because LN680851 available sequence is only 561 nt or 187 aa. aDenotes PRV “isolates” from coho salmon; bDenotes PRV “isolate” from Chinook salmon; cDenotes PRV “isolate” from sea trout. dDenotes PRV “isolate” from rainbow trout