Table 4.

p53 detection analysis

Sample ID	Anatomical site	L1 DNA	HPV 16 E6/E7 DNA	HPV 16 mRNA	p53 PCR	Smokers
2	Oropharynx	6	–	–	–	+
5	Larynx	16	–	–	–	–
7	Hypopharynx	–	–	–	–	+
8	Other	–	–	–	–	+
9	Oropharynx	6, 16	–	–	–	+
12	Other	6, 11	–	–	–	–
17	Oropharynx	Mixed	–	–	–	+
18	Larynx	–	–	–	–	+
26	Larynx	6	–	–	+	–
39	Oropharynx	16	–	–	+	–
50	Oropharynx	16	+	–	–	+
52	Hypopharynx	16	+	–	–	–
10	Larynx	6	–	+	–	–
14	Larynx	–	–	+	–	+
15	Larynx	–	+	+	–	–
16	Oral cavity	40	–	+	+	–
33	Larynx	16	+	+	+	+
35	Hypopharynx	16	–	+	+	–
36	Larynx	16	+	+	+	+
41	Oropharynx	16, mixed	+	+	+	–
54	Larynx	–	–	+	+	–

Amplicons spanning the entire p53 open reading frame were amplified from 6/9 HPV 16 E6/E7 mRNA positive HNSCCs (bottom), but only 2/12 transcript negative samples (top) (p = 0.032, Fisher’s exact test; OR 10). HPV16 DNA or P16^INK4a showed no relationship with p53 PCR