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. 2016 Feb 8;2(2):93–103. doi: 10.1002/cjp2.38

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© 2016 John Wiley and Sons Ltd and The Pathological Society of Great Britain and Ireland

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Impact of blood contamination on miRNA expression signatures. (A) Representative examples of miRNA expression levels (Ct) according to the percentage of blood contamination. Nucleic acids from resected thyroid samples (0%) were mixed with nucleic acids from blood samples (100%) to mimic clinical samples contaminated with 10, 25, 50 or 75% of blood. miR‐144‐5p was highly over expressed in blood relative to resected thyroid tissue (ΔCt=Ct _0% − Ct _100% >10), which was confirmed by microarray differential analyses (data not shown). (B) Relationship between miRNA expression difference in whole blood relative to resected thyroid tissues determined in Figure 2A (ΔCt, y axis) and the correlation between the Ct of each miRNA with the Ct of miR‐144‐5p measured in 235 preoperative thyroid nodule aspirates (R ², x axis). In this experiment, the correlation of a given miRNA with miR‐144‐5p in nodule aspirates is taken as a proxy for the degree to which the miRNA's expression measurement is influenced by blood contamination. Abbreviation: FNA, fine‐needle aspiration.