FIG 7.

Regulation of HIF-α-independent genes by B-Myb. (A) Scatter plots of total probes examined by microarray. Total RNA purified from control or B-Myb knockdown 1 or 2 cells was used for microarray analysis to identify genes differentially expressed in response to B-Myb knockdown. The solid line indicates identical gene expression. The dashed lines indicate the threshold defined as 2 or 1/2 times expression. (B) The 370 B-Myb-regulated genes are grouped by coding or noncoding and by upregulated or downregulated. (C) The 233 B-Myb-regulated coding genes are grouped by conceivable biological functions. (D) Upregulation of CFB, FKBP1B, and SEPT6 by knockdown of B-Myb. Total RNA was purified from control or B-Myb knockdown (B-Myb#1 or B-Myb#2) cells and analyzed by quantitative RT-PCR. *, P < 0.05; **, P < 0.01.