FIG 6.

Artificial recruitment of Cdc13 to the SiRTA 5L-35 stimulatory site increases the rate of GCR formation. (A) The SiRTA 5L-35 Stim sequence was replaced with two tandem copies of the Gal4 upstream activating sequence (2× UAS) or with a string of adenines [poly(A); identical sequence to that of mutation d in Fig. 2A]. (B) The rate of GCR formation within SiRTA 5L-35 is shown for strains containing either the 2× UAS or poly(A) sequences integrated in place of SiRTA 5L-35 Stim. Cells are transformed with pRS314 (empty vector) or with pRS414 expressing either CDC13 or RAP1 as N-terminal fusions with the Gal4 DNA binding domain (GBD). Values for the three rightmost columns are maximum estimates (see Materials and Methods). Error bars indicate standard deviations for three independent experiments. Averages indicated with 2 asterisks are significantly different (P < 0.01) by ANOVA with post hoc Tukey's HSD.