FIG 2.

Lytic activity of SltF. (A) SltF (2 μM) was incubated at ambient temperature with M. luteus cells suspended in 50 mM sodium phosphate buffer (pH 7.0) alone (●) or in the presence of 2 μM FlgB (■) or 2 μM FlgF (▲). Shown are representative curves for the progress of lysis as monitored turbidimetrically at OD₅₉₅. ◆, 2 μM SltF(Glu57Ala) assayed in the absence of other added protein. Negative controls: ○, no enzyme added; □, FlgB; △, FlgF alone. (B) pH activity profile of SltF (error bars denote standard errors [SE]; n = 3).