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Localization of GFP-FlgZ is impacted by c-di-GMP levels and MotCD. (A) Representative images of WT, ΔbifA, and ΔmotCD strains expressing GFP-FlgZ. (B) For each strain, more than 2,000 bacteria from three independent experiments were analyzed, and values are reported as means ± SEM. Significance was determined by analysis of variance and Dunnett's posttest comparison for differences relative to WT. ***, P < 0.001.
