TABLE 3.

K^s phenotype of the ΔptsN mutation and its suppression by absence of YcgO in K⁺ transporter-defective backgrounds

Strain	Relevant genotype	Growth rate (h−1) on mediuma:
		K1	K20	K40	K115
MC4100b	kdp+ kup+trkA+	0.86	0.88 ± 0.01	0.88 ± 0.01	0.88 ± 0.01
JD17b	kdp+ kup+ trkA+ ΔptsN	0.83 ± 0.01	0.69 ± 0.01	0.60 ± 0.02	0.63 ± 0.03
JD466	kdp+ kup+ trkA+ ΔycgO	0.91 ± 0.01	0.89 ± 0.02	0.91 ± 0.02	0.90 ± 0.03
JD509	kdp+ kup+ trkA+ ΔptsN ΔycgO	0.83 ± 0.02	0.80 ± 0.02	0.82	0.84
JD656b	ΔkdpA kup+ trkA+	0.84 ± 0.02	0.85 ± 0.02	0.85 ± 0.02	0.83 ± 0.02
JD662b	ΔkdpA kup+ trkA+ ΔptsN	0.64 ± 0.02	0.66 ± 0.01	0.55 ± 0.02	0.58 ± 0.01
JD694	ΔkdpA kup+ trkA+ ΔycgO	0.85 ± 0.01	0.89 ± 0.02	0.89 ± 0.01	0.89
JD725	ΔkdpA kup+ trkA+ ΔptsN ΔycgO	0.70 ± 0.06	0.81 ± 0.02	0.82	0.82
JD657b,c	ΔkdpA kup trkA	<0.05	<0.05	0.67 ± 0.01	0.83 ± 0.01
JD658b,c	ΔkdpA kup trkA ΔptsN	<0.05	<0.05	<0.05	0.46 ± 0.02
JD693	ΔkdpA kup trkA ΔycgO	<0.05	<0.05	0.72 ± 0.03	0.86 ± 0.01
JD714c	ΔkdpA kup trkA ΔptsN ΔycgO	<0.05	<0.05	0.76 ± 0.24	0.88 ± 0.01

^aShown are growth rates (means ± standard errors) of three quartets of strains in K₁, K₂₀, K₄₀, and K₁₁₅ media. The first quartet, MC4100 to JD590, bears all K⁺ uptake systems, the second, JD656 to JD725, lacks the Kdp transporter, and the third, JD657 to JD714, lacks all K⁺ uptake systems. Growth rates for the quartet with Kdp as the sole K⁺ uptake system are displayed in Fig. 4. The PtsN, YcgO, and K⁺ transporter status with respect to Kdp, Trk, and Kup in each quartet is indicated.

^bValues for growth rates from Fig. 1.

^cContains the zha-203::Tn10 marker.