. 2016 Jun 13;198(13):1868–1882. doi: 10.1128/JB.01029-15

TABLE 4.

Reduction in the cellular K⁺ content by overexpression of ycgO in the parent and its restoration by overexpression of kup

Strain	Relevant genotype	Relative K⁺ content after growth in medium^a:
Strain	Relevant genotype	K₁	K₄₀	K₁₁₅
JD728	kdp⁺ kup trkA attλ::(P_trc99A bla)	1 (0.18)	1 (0.12)	1 (0.01)
JD729	kdp⁺ kup trkA attλ::(P_trcycgO bla)	0.73 (0.14)	0.62 (0.04)	0.57 (0.7)
JD732/pHYD3025^b	kdp⁺ kup trkA attλ::(P_trcycgO bla::Kan)	0.9 (0.11)	0.8 (0.16)	0.71 (0.08)
JD732/pHYD1852^c	kdp⁺ kup trkA attλ::(P_trcycgO bla::Kan)	1.14 (0.02)	1.33 (0.04)	1.07 (0.04)

The K⁺ content obtained for strain JD728 bearing integration of the plasmid pTrc99A in attB [attλ::(P_trc99A bla)] in K₁, K₄₀, and K₁₁₅ media was set at 100% = 1, and the relative K⁺ contents for the other strains are indicated. K⁺ content was determined following 2 h of growth of the indicated strains in K₁, K₄₀, and K₁₁₅ media containing 0.1 mM IPTG. The relative standard deviations (SD) for the measurements are indicated in parentheses. The K⁺ contents (mean ± SD) for JD728 following its growth in K₁, K₄₀, and K₁₁₅ media were 162 ± 29.4, 147 ± 17.3, and 229 ± 3.4 nmol K⁺/A₆₀₀, respectively.

Vector.

Plasmid bearing kup under the expression control of the P_trc promoter.